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expression constructs pef1a hopn4s mcherry  (Addgene inc)


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    Structured Review

    Addgene inc expression constructs pef1a hopn4s mcherry
    Light stimulation was applied to Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe via an optic fiber (3 μW from the fiber tip, 473-nm, 6 h from time 0). Heart rate and core body temperature are also simultaneously taken by telemetry sensors. Video plays at 60x speed. Images in Figure 1D are created from this video.
    Expression Constructs Pef1a Hopn4s Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+constructs+pef1a+hopn4s+mcherry/pmc09701604-468-34-39?v=Addgene+inc
    Average 91 stars, based on 2 article reviews
    expression constructs pef1a hopn4s mcherry - by Bioz Stars, 2026-07
    91/100 stars

    Images

    1) Product Images from "Optogenetic induction of hibernation-like state with modified human Opsin4 in mice"

    Article Title: Optogenetic induction of hibernation-like state with modified human Opsin4 in mice

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2022.100336

    Light stimulation was applied to Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe via an optic fiber (3 μW from the fiber tip, 473-nm, 6 h from time 0). Heart rate and core body temperature are also simultaneously taken by telemetry sensors. Video plays at 60x speed. Images in Figure 1D are created from this video.
    Figure Legend Snippet: Light stimulation was applied to Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe via an optic fiber (3 μW from the fiber tip, 473-nm, 6 h from time 0). Heart rate and core body temperature are also simultaneously taken by telemetry sensors. Video plays at 60x speed. Images in Figure 1D are created from this video.

    Techniques Used:

    Representative thermal and visual imaging of a Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe, during optogenetic stimulation via a blue LED attached on the skull (250 μW, 1 h from time 0). Core body temperature was simultaneously taken by telemetry system. Video plays at 60x speed. Images in Figure 4B are created from this video.
    Figure Legend Snippet: Representative thermal and visual imaging of a Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe, during optogenetic stimulation via a blue LED attached on the skull (250 μW, 1 h from time 0). Core body temperature was simultaneously taken by telemetry system. Video plays at 60x speed. Images in Figure 4B are created from this video.

    Techniques Used:



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    Addgene inc expression constructs pef1a hopn4s mcherry
    Light stimulation was applied to Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe via an optic fiber (3 μW from the fiber tip, 473-nm, 6 h from time 0). Heart rate and core body temperature are also simultaneously taken by telemetry sensors. Video plays at 60x speed. Images in Figure 1D are created from this video.
    Expression Constructs Pef1a Hopn4s Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+constructs+pef1a+hopn4s+mcherry/pmc09701604-468-34-39?v=Addgene+inc
    Average 91 stars, based on 1 article reviews
    expression constructs pef1a hopn4s mcherry - by Bioz Stars, 2026-07
    91/100 stars
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    Light stimulation was applied to Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe via an optic fiber (3 μW from the fiber tip, 473-nm, 6 h from time 0). Heart rate and core body temperature are also simultaneously taken by telemetry sensors. Video plays at 60x speed. Images in Figure 1D are created from this video.

    Journal: Cell Reports Methods

    Article Title: Optogenetic induction of hibernation-like state with modified human Opsin4 in mice

    doi: 10.1016/j.crmeth.2022.100336

    Figure Lengend Snippet: Light stimulation was applied to Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe via an optic fiber (3 μW from the fiber tip, 473-nm, 6 h from time 0). Heart rate and core body temperature are also simultaneously taken by telemetry sensors. Video plays at 60x speed. Images in Figure 1D are created from this video.

    Article Snippet: Stimulating light for hOPN4s and excitation light for fluorescence were the same (480 nm, 500 ms, every 2 s), as described in a previous study., For bioluminescence-based Ca2+ recording, cells were transfected with the expression constructs pEF1a-hOPN4s-mCherry and pcDNA3-Nano-lantern(Ca2+)_600 (Addgene, #51982 ).

    Techniques:

    Representative thermal and visual imaging of a Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe, during optogenetic stimulation via a blue LED attached on the skull (250 μW, 1 h from time 0). Core body temperature was simultaneously taken by telemetry system. Video plays at 60x speed. Images in Figure 4B are created from this video.

    Journal: Cell Reports Methods

    Article Title: Optogenetic induction of hibernation-like state with modified human Opsin4 in mice

    doi: 10.1016/j.crmeth.2022.100336

    Figure Lengend Snippet: Representative thermal and visual imaging of a Qrfp-iCre mouse expressing hOPN4dC-mCherry in the AVPe, during optogenetic stimulation via a blue LED attached on the skull (250 μW, 1 h from time 0). Core body temperature was simultaneously taken by telemetry system. Video plays at 60x speed. Images in Figure 4B are created from this video.

    Article Snippet: Stimulating light for hOPN4s and excitation light for fluorescence were the same (480 nm, 500 ms, every 2 s), as described in a previous study., For bioluminescence-based Ca2+ recording, cells were transfected with the expression constructs pEF1a-hOPN4s-mCherry and pcDNA3-Nano-lantern(Ca2+)_600 (Addgene, #51982 ).

    Techniques: